https://doi.org/10.1016/j.tiv.2019.03.004
Bruna Ferreira Tollstadius, Artur Christian Garcia da Silva, Bruna Cristiane Oliveira Pedralli, Marize Campos Valadares
Laboratory of Education and Research in In vitro Toxicology – ToxIn, Faculty of Pharmacy, Universidade Federal de Goiás, Goiânia, GO, Brazil
In this study, researchers evaluated the effects of the fungicide carbendazim using a reconstructed human alveolar epithelial model. The compound was aerosolized and applied via the VITROCELL® Cloud 12 exposure system, allowing for realistic air-liquid interface conditions. While traditional 2D cell cultures showed minimal response, the 3D alveolar model revealed pronounced cytotoxic effects, including cell death, activation of caspase-3, α-tubulin disruption, and increased ROS production.
