https://doi.org/10.1016/j.fct.2022.113457
Jaqueline Rodrigues da Penha a, Artur Christian Garcia da Silva a, Renato Ivan de Ávila b, Marize Campos Valadares a
a Laboratory of Education and Research in In vitro Toxicology, Faculty of Pharmacy, Universidade Federal de Goiás, Goiânia, GO, Brazil
b Department of Immunotechnology, Lund University, Medicon Village, Lund, Sweden
This study established an air–liquid interface (ALI) corneal model using bovine eye explants to assess ocular toxicity of ten chemicals and seven hair straightening mixtures. The model remained viable for six days and distinguished between severe (GHS Cat. 1) and moderate (Cat. 2) irritants. Chemical mixtures reduced tissue viability and caused histopathological changes, especially after aerosol exposure, which provided more realistic toxicity data. Increased ROS levels correlated with tissue damage, indicating oxidative stress involvement. Some mixtures caused reversible effects, highlighting the model’s usefulness as a simple, cost-effective tool to study corneal injury and recovery mechanisms.
