https://doi.org/10.1016/j.fct.2022.112820
Tatyane Gonçalves Hayasaki, Thaís Rosa Marques Santos, Artur Christian Garcia Silva, Marize Campos Valadares
Laboratory of Education and Research In Vitro Toxicology (Tox In), Faculty of Pharmacy, Universidade Federal de Goiás, Goiânia, Goiás, Brazil
This study presents an ex vivo bovine bronchial model for assessing pulmonary toxicity. Exposure to paraformaldehyde (PFA) via liquid and aerosol routes revealed concentration-dependent decreases in viability, especially after aerosol exposure, and induced biomarkers such as altered epithelial thickness, mitochondrial activity, ROS generation, and caspase-3 activation. Additionally, exposure to chemicals from various UN GHS toxicity categories produced consistent concentration-dependent responses. The model offers a cost-effective and reproducible platform for evaluating respiratory toxicity and serves as a promising non-animal alternative for chemical testing.
